Laurent Griffe

Analytical Methods: Multi-nuclear FT-NMR ▪ FT-IR ▪ UV-vis ▪ Mass Spectrometry (MALDI for peptide)Purification skills:HPLC (C18 / Peptide separation) ▪ crystallization ▪ precipitation ▪ distillation ▪ sublimation ▪ chromatography (normal, reverse phase)Synthetic skills:Heterocyclic ▪ organometallic ▪ macromolecular ▪ phosphorus chemistry ▪Heterogenous and homogenous catalysis ▪ Peptide chemistry ▪ Diastereoselective reactions ▪From mg to kg scale in non-GMP or GMP environment ▪ Schlenk line and Vacuum/Argon techniques ▪ Glove Box ▪ Technology transfer to pilot lab. and scale-up:Reaction calorimetry (Algochem) ▪ DSC ▪ ATG-ATDProteins Biochemistry:Enzymatic synthesis ▪ dialysis ▪ ion exchange HPLC ▪ purification and concentration with AMICON tubesBiology Analytical Methods:Flow cytometry acquisition and analysis with FACScan on LSR-II with FACSDiva and Cell Quest environmentsCell culture:Normal/tumoral cell culture ▪ neurons and glia cell culture ▪ basic training to work with animal (scientific procedure): ratComputer skills:Word ▪ Power Point ▪ Excel ▪ technology forecasting using bibliography and international patent databases (SciFinder, Beilstein, Gmelin, Reaxys)