myriam adam

COMPETENCES: -Poly A+ and total RNA extraction, DNA extraction, RT-PCR, semi-quantitative PCR,qPCR, LMPCR, 5'-RACE, sequencing, Northern blot, Southern blot, screening ofgenomic and cDNA libraries, plasmid construction, microarray technology ( Affymetrixand filters), RNA-EMSA, protein analysis (Western blot, enzymatic assays,immunoprecipitation), production of recombinant proteins, ELISA test-Cell culturing (monolayer and suspension), transfections (bacteria, yeasts, algae,Dictyostelium, mouse and human cell lines), retrovirus and lentivirus production, bonemarrow transplant, immunofluorescence staining, unicellular microorganismsmutagenesis (insertional, UV, chemical), random spore plating analysis and tetradanalysis (algae)-Bio informatics, GeneSpring Microarray analysis, Microsoft programs, Scientificprograms (Sigma plot, Macplasm, Plasmid Artist